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superscript iv one-step rt-pcr kit  (Thermo Fisher)


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    Thermo Fisher superscript iv one-step rt-pcr kit
    Superscript Iv One Step Rt Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iv+one-step+rt-pcr+kit/high+capacity+cdna+reverse+transcription+kit/pmc12218770-445-20-25
    Average 90 stars, based on 1 article reviews
    superscript iv one-step rt-pcr kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Entacapone’s therapeutic potential in renal fibrosis by targeting miR-34a/TGFβ1/Smad2 signaling: an experimental rat model study
    Article Snippet: .. Subsequently, reverse transcription was carried out using the SuperScript IV One-Step RT-PCR kit (Cat# 12,594,100, Thermo Fisher Scientific, Waltham, MA, USA) on a 96-well StepOne instrument (Applied Biosystems, USA). ..

    Article Title: Ameliorative Effects of Blue LED Irradiated and Non-Irradiated Fenugreek Seed Extracts on Murine Trichinosis
    Article Snippet: According to the manufacturer's instructions, total RNA was extracted from muscular tissue lysates using a Direct-zol RNA Miniprep Plus kit (ZYMO RESEARCH CORP., USA). .. Reverse transcription of the extracted RNA (cDNA synthesis) was performed using the Superscript IV One-Step RT-PCR Kit (Thermo Fisher Scientific, Waltham, MA, USA), followed by PCR amplification in a single reaction tube using BAX gene primers (5′-ACTCCCATTCTTCCACCTTTG-3′ and 5′-CCCTGTTGCTGTAGCCATATT-3′) and the internal control primers for GAPDH (5′-TGGATTTGGACGCATTGGTC-3′ and 5′-TTTGCACTGGTACGTGTTGAT-3′). .. The prepared reaction mix samples were analyzed using real-time PCR (StepOne, Applied Biosystems, Foster City, USA).

    Article Title: Adropin contributes to the nephro-protective effect of vitamin D in renal aging in a rat model via MAPK/HIFα/VEGF/eNOS mechanism.
    Article Snippet: Great efforts have been established to promote healthy aging and prevent illnesses connected to aging.. The prevalence of elderly population increases and this will be associated with an increase in disability and illness.. This study analyzed renal aging process following administration of Dgalactose (120 mg/kg/day ip for 8 weeks), and the potential protective effects of vitamin D administration (1000 and 10000 IU/kg/day by oral gavage for 8 weeks) in animals with Dgalactose induced aging.

    Article Title: Effect of Gamma Rays on Adropin as a Potential Hepatokine Marker for Liver Damage in Male Albino Rats
    Article Snippet: Total ribonucleic acid was extracted from hydrolyzed tissues with Direct-zol RNA Miniprep Plus (Cat# R2072, ZYMO RESEARCH CORP. USA) and then the amount was evaluated by Beckman dual spectrophotometer (USA). .. Superscript IV One-Step RT-PCR kit (Cat# 12594100, Thermo Fisher Scientific, Waltham, MA USA) was applied for reverse transcription of the extracted RNA and then by PCR in the same step. ..

    Article Title: Design, synthesis and characterization of novel pyridazin-3-one derivatives: in vitro vasorelaxant activity and in silico insights into eNOS modulation
    Article Snippet: For the eNOS mRNA expression assay, Total RNA was extracted from aortic tissue lysate with Direct-zol RNA Miniprep Plus (cat# R2072, Zymo Research Corp. USA) and then quantity and quality were assessed by a Beckman dual spectrophotometer (USA). .. Optical density (OD) of extracted RNA was measured at 260 nm ultraviolet wavelength to ensure RNA purification, then SuperScript IV One-Step RT-PCR kit (Cat# 12594100, Thermo Fisher Scientific, Waltham, MA, USA) was utilized for reverse transcription of extracted RNA, followed by PCR in one step. ..

    Article Title: Octreotide attenuates intestinal ischemia/reperfusion mischief in rats through modulation of Nrf2/PRX2/ASK1/JNK signaling pathway.
    Article Snippet: Total RNA was extracted from homogenized tissues from all the different groups using Direct-zol RNA Miniprep Plus (Zymo Research Co., Ltd., USA, Cat# R2072), after which quantity and quality of the RNA were assessed via a Beckman dual spectrophotometer (USA). .. A SuperScript IV One-Step RT‒PCR Kit (Thermo Fisher Scientific, MA, USA, Cat# 12594100) was used for reverse transcription of the extracted RNA followed by PCR. .. A 48-well plate Step One instrument (Applied Biosystems, USA) was used for thermal profiling as follows: 10 min at 45 °C for reverse transcription, 2 min at 98 °C for RT inactivation, and initial denaturation for 40 cycles of 10 s at 98 °C, 10 s at 55 °C, and 30 s at 72 °C for the amplification step.

    One Step RT-PCR:

    Article Title: Entacapone’s therapeutic potential in renal fibrosis by targeting miR-34a/TGFβ1/Smad2 signaling: an experimental rat model study
    Article Snippet: .. Subsequently, reverse transcription was carried out using the SuperScript IV One-Step RT-PCR kit (Cat# 12,594,100, Thermo Fisher Scientific, Waltham, MA, USA) on a 96-well StepOne instrument (Applied Biosystems, USA). ..

    Article Title: Ameliorative Effects of Blue LED Irradiated and Non-Irradiated Fenugreek Seed Extracts on Murine Trichinosis
    Article Snippet: According to the manufacturer's instructions, total RNA was extracted from muscular tissue lysates using a Direct-zol RNA Miniprep Plus kit (ZYMO RESEARCH CORP., USA). .. Reverse transcription of the extracted RNA (cDNA synthesis) was performed using the Superscript IV One-Step RT-PCR Kit (Thermo Fisher Scientific, Waltham, MA, USA), followed by PCR amplification in a single reaction tube using BAX gene primers (5′-ACTCCCATTCTTCCACCTTTG-3′ and 5′-CCCTGTTGCTGTAGCCATATT-3′) and the internal control primers for GAPDH (5′-TGGATTTGGACGCATTGGTC-3′ and 5′-TTTGCACTGGTACGTGTTGAT-3′). .. The prepared reaction mix samples were analyzed using real-time PCR (StepOne, Applied Biosystems, Foster City, USA).

    Article Title: Adropin contributes to the nephro-protective effect of vitamin D in renal aging in a rat model via MAPK/HIFα/VEGF/eNOS mechanism.
    Article Snippet: Great efforts have been established to promote healthy aging and prevent illnesses connected to aging.. The prevalence of elderly population increases and this will be associated with an increase in disability and illness.. This study analyzed renal aging process following administration of Dgalactose (120 mg/kg/day ip for 8 weeks), and the potential protective effects of vitamin D administration (1000 and 10000 IU/kg/day by oral gavage for 8 weeks) in animals with Dgalactose induced aging.

    Article Title: Piceatannol protects against cisplatin-induced ovarian toxicity in rats via modulation of PTEN/PI3 K/AKT axis.
    Article Snippet: Premenopausal women diagnosed with cancer may suffer from ovarian toxicity, which affects their quality of life.. Cisplatin (CIS) is an effective chemotherapeutic drug used for different types of tumors.. However, CIS has been reported to cause ovarian toxicity.

    Article Title: Effect of Gamma Rays on Adropin as a Potential Hepatokine Marker for Liver Damage in Male Albino Rats
    Article Snippet: Total ribonucleic acid was extracted from hydrolyzed tissues with Direct-zol RNA Miniprep Plus (Cat# R2072, ZYMO RESEARCH CORP. USA) and then the amount was evaluated by Beckman dual spectrophotometer (USA). .. Superscript IV One-Step RT-PCR kit (Cat# 12594100, Thermo Fisher Scientific, Waltham, MA USA) was applied for reverse transcription of the extracted RNA and then by PCR in the same step. ..

    Article Title: Enhanced Therapeutic Efficacy of Omeprazole Nanosuspension in Ethanol-Induced Gastric Ulcer: A Focus on Oxidative Stress and Inflammatory Pathways.
    Article Snippet: Reverse transcription into cDNA was conducted using the InvitrogenTM SuperScriptTM IV One-Step RT-PCR System from Thermo Fisher Scientific, Waltham, MA, USA, in accordance with the manufacturer’s guidelines. .. The expression levels of target genes in each sample were evaluated using the SuperScript IV One-Step RT-PCR kit (Cat# 12594100, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Design, synthesis and characterization of novel pyridazin-3-one derivatives: in vitro vasorelaxant activity and in silico insights into eNOS modulation
    Article Snippet: For the eNOS mRNA expression assay, Total RNA was extracted from aortic tissue lysate with Direct-zol RNA Miniprep Plus (cat# R2072, Zymo Research Corp. USA) and then quantity and quality were assessed by a Beckman dual spectrophotometer (USA). .. Optical density (OD) of extracted RNA was measured at 260 nm ultraviolet wavelength to ensure RNA purification, then SuperScript IV One-Step RT-PCR kit (Cat# 12594100, Thermo Fisher Scientific, Waltham, MA, USA) was utilized for reverse transcription of extracted RNA, followed by PCR in one step. ..

    cDNA Synthesis:

    Article Title: Ameliorative Effects of Blue LED Irradiated and Non-Irradiated Fenugreek Seed Extracts on Murine Trichinosis
    Article Snippet: According to the manufacturer's instructions, total RNA was extracted from muscular tissue lysates using a Direct-zol RNA Miniprep Plus kit (ZYMO RESEARCH CORP., USA). .. Reverse transcription of the extracted RNA (cDNA synthesis) was performed using the Superscript IV One-Step RT-PCR Kit (Thermo Fisher Scientific, Waltham, MA, USA), followed by PCR amplification in a single reaction tube using BAX gene primers (5′-ACTCCCATTCTTCCACCTTTG-3′ and 5′-CCCTGTTGCTGTAGCCATATT-3′) and the internal control primers for GAPDH (5′-TGGATTTGGACGCATTGGTC-3′ and 5′-TTTGCACTGGTACGTGTTGAT-3′). .. The prepared reaction mix samples were analyzed using real-time PCR (StepOne, Applied Biosystems, Foster City, USA).

    Polymerase Chain Reaction:

    Article Title: Ameliorative Effects of Blue LED Irradiated and Non-Irradiated Fenugreek Seed Extracts on Murine Trichinosis
    Article Snippet: According to the manufacturer's instructions, total RNA was extracted from muscular tissue lysates using a Direct-zol RNA Miniprep Plus kit (ZYMO RESEARCH CORP., USA). .. Reverse transcription of the extracted RNA (cDNA synthesis) was performed using the Superscript IV One-Step RT-PCR Kit (Thermo Fisher Scientific, Waltham, MA, USA), followed by PCR amplification in a single reaction tube using BAX gene primers (5′-ACTCCCATTCTTCCACCTTTG-3′ and 5′-CCCTGTTGCTGTAGCCATATT-3′) and the internal control primers for GAPDH (5′-TGGATTTGGACGCATTGGTC-3′ and 5′-TTTGCACTGGTACGTGTTGAT-3′). .. The prepared reaction mix samples were analyzed using real-time PCR (StepOne, Applied Biosystems, Foster City, USA).

    Article Title: Effect of Gamma Rays on Adropin as a Potential Hepatokine Marker for Liver Damage in Male Albino Rats
    Article Snippet: Total ribonucleic acid was extracted from hydrolyzed tissues with Direct-zol RNA Miniprep Plus (Cat# R2072, ZYMO RESEARCH CORP. USA) and then the amount was evaluated by Beckman dual spectrophotometer (USA). .. Superscript IV One-Step RT-PCR kit (Cat# 12594100, Thermo Fisher Scientific, Waltham, MA USA) was applied for reverse transcription of the extracted RNA and then by PCR in the same step. ..

    Article Title: Design, synthesis and characterization of novel pyridazin-3-one derivatives: in vitro vasorelaxant activity and in silico insights into eNOS modulation
    Article Snippet: For the eNOS mRNA expression assay, Total RNA was extracted from aortic tissue lysate with Direct-zol RNA Miniprep Plus (cat# R2072, Zymo Research Corp. USA) and then quantity and quality were assessed by a Beckman dual spectrophotometer (USA). .. Optical density (OD) of extracted RNA was measured at 260 nm ultraviolet wavelength to ensure RNA purification, then SuperScript IV One-Step RT-PCR kit (Cat# 12594100, Thermo Fisher Scientific, Waltham, MA, USA) was utilized for reverse transcription of extracted RNA, followed by PCR in one step. ..

    Article Title: Octreotide attenuates intestinal ischemia/reperfusion mischief in rats through modulation of Nrf2/PRX2/ASK1/JNK signaling pathway.
    Article Snippet: Total RNA was extracted from homogenized tissues from all the different groups using Direct-zol RNA Miniprep Plus (Zymo Research Co., Ltd., USA, Cat# R2072), after which quantity and quality of the RNA were assessed via a Beckman dual spectrophotometer (USA). .. A SuperScript IV One-Step RT‒PCR Kit (Thermo Fisher Scientific, MA, USA, Cat# 12594100) was used for reverse transcription of the extracted RNA followed by PCR. .. A 48-well plate Step One instrument (Applied Biosystems, USA) was used for thermal profiling as follows: 10 min at 45 °C for reverse transcription, 2 min at 98 °C for RT inactivation, and initial denaturation for 40 cycles of 10 s at 98 °C, 10 s at 55 °C, and 30 s at 72 °C for the amplification step.

    Amplification:

    Article Title: Ameliorative Effects of Blue LED Irradiated and Non-Irradiated Fenugreek Seed Extracts on Murine Trichinosis
    Article Snippet: According to the manufacturer's instructions, total RNA was extracted from muscular tissue lysates using a Direct-zol RNA Miniprep Plus kit (ZYMO RESEARCH CORP., USA). .. Reverse transcription of the extracted RNA (cDNA synthesis) was performed using the Superscript IV One-Step RT-PCR Kit (Thermo Fisher Scientific, Waltham, MA, USA), followed by PCR amplification in a single reaction tube using BAX gene primers (5′-ACTCCCATTCTTCCACCTTTG-3′ and 5′-CCCTGTTGCTGTAGCCATATT-3′) and the internal control primers for GAPDH (5′-TGGATTTGGACGCATTGGTC-3′ and 5′-TTTGCACTGGTACGTGTTGAT-3′). .. The prepared reaction mix samples were analyzed using real-time PCR (StepOne, Applied Biosystems, Foster City, USA).

    Control:

    Article Title: Ameliorative Effects of Blue LED Irradiated and Non-Irradiated Fenugreek Seed Extracts on Murine Trichinosis
    Article Snippet: According to the manufacturer's instructions, total RNA was extracted from muscular tissue lysates using a Direct-zol RNA Miniprep Plus kit (ZYMO RESEARCH CORP., USA). .. Reverse transcription of the extracted RNA (cDNA synthesis) was performed using the Superscript IV One-Step RT-PCR Kit (Thermo Fisher Scientific, Waltham, MA, USA), followed by PCR amplification in a single reaction tube using BAX gene primers (5′-ACTCCCATTCTTCCACCTTTG-3′ and 5′-CCCTGTTGCTGTAGCCATATT-3′) and the internal control primers for GAPDH (5′-TGGATTTGGACGCATTGGTC-3′ and 5′-TTTGCACTGGTACGTGTTGAT-3′). .. The prepared reaction mix samples were analyzed using real-time PCR (StepOne, Applied Biosystems, Foster City, USA).

    Spectrophotometry:

    Article Title: Adropin contributes to the nephro-protective effect of vitamin D in renal aging in a rat model via MAPK/HIFα/VEGF/eNOS mechanism.
    Article Snippet: Great efforts have been established to promote healthy aging and prevent illnesses connected to aging.. The prevalence of elderly population increases and this will be associated with an increase in disability and illness.. This study analyzed renal aging process following administration of Dgalactose (120 mg/kg/day ip for 8 weeks), and the potential protective effects of vitamin D administration (1000 and 10000 IU/kg/day by oral gavage for 8 weeks) in animals with Dgalactose induced aging.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Piceatannol protects against cisplatin-induced ovarian toxicity in rats via modulation of PTEN/PI3 K/AKT axis.
    Article Snippet: Premenopausal women diagnosed with cancer may suffer from ovarian toxicity, which affects their quality of life.. Cisplatin (CIS) is an effective chemotherapeutic drug used for different types of tumors.. However, CIS has been reported to cause ovarian toxicity.

    Expressing:

    Article Title: Enhanced Therapeutic Efficacy of Omeprazole Nanosuspension in Ethanol-Induced Gastric Ulcer: A Focus on Oxidative Stress and Inflammatory Pathways.
    Article Snippet: Reverse transcription into cDNA was conducted using the InvitrogenTM SuperScriptTM IV One-Step RT-PCR System from Thermo Fisher Scientific, Waltham, MA, USA, in accordance with the manufacturer’s guidelines. .. The expression levels of target genes in each sample were evaluated using the SuperScript IV One-Step RT-PCR kit (Cat# 12594100, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Purification:

    Article Title: Design, synthesis and characterization of novel pyridazin-3-one derivatives: in vitro vasorelaxant activity and in silico insights into eNOS modulation
    Article Snippet: For the eNOS mRNA expression assay, Total RNA was extracted from aortic tissue lysate with Direct-zol RNA Miniprep Plus (cat# R2072, Zymo Research Corp. USA) and then quantity and quality were assessed by a Beckman dual spectrophotometer (USA). .. Optical density (OD) of extracted RNA was measured at 260 nm ultraviolet wavelength to ensure RNA purification, then SuperScript IV One-Step RT-PCR kit (Cat# 12594100, Thermo Fisher Scientific, Waltham, MA, USA) was utilized for reverse transcription of extracted RNA, followed by PCR in one step. ..



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